In the winter of 1532, Henry attended a meeting with Francis I of France at Calais in which he enlisted the support of the French king for his new marriage.[62] Immediately upon returning to Dover in England, Henry and Anne went through a secret wedding service.[63] She soon became pregnant and there was a second wedding service in London on 25 January 1533. On 23 May 1533, Cranmer, sitting in judgment at a special court convened at Dunstable Priory to rule on the validity of the king's marriage to Catherine of Aragon, declared the marriage of Henry and Catherine null and void. Five days later, on 28 May 1533, Cranmer declared the marriage of Henry and Anne to be valid.[64] Catherine was formally stripped of her title as queen, and Anne was crowned queen consort on 1 June 1533. The queen gave birth to a daughter slightly prematurely on 7 September 1533. The child was christened Elizabeth, in honour of Henry's mother, Elizabeth of York.[65] Rejecting the decisions of the Pope, Parliament validated the marriage of Henry and Anne with the First Succession Act (Act of Succession 1533). Catherine's daughter, Mary, was declared illegitimate, and Anne's issue were declared next in the line of succession. Most notable in this declaration was a clause repudiating "any foreign authority, prince or potentate". All adults in the Kingdom were required to acknowledge the Act's provisions by oath; those who refused were subject to imprisonment for life. Any publisher or printer of any literature alleging that the marriage was invalid was automatically guilty of high treason and could be punished by death.

Catherine was formally stripped of her title as Queen and Anne was consequently crowned queen consort on 1 June 1533 in a magnificent ceremony at Westminster Abbey with a banquet afterwards.[74] She was the last Queen Consort of England to be crowned separately from her husband. Unlike any other queen consort, Anne was crowned with St Edward's crown, which had previously been used to crown only a reigning monarch.[75] Historian Alice Hunt suggests that this was done because Anne's pregnancy was visible by then and she was carrying the heir who was presumed to be male.[76] On the previous day, Anne had taken part in an elaborate procession through the streets of London seated in a litter of "white cloth of gold" that rested on two palfreys clothed to the ground in white damask, while the barons of the Cinque Ports held a canopy of cloth of gold over her head. In accordance with tradition she wore white, and on her head a gold coronet beneath which her long dark hair hung down freely.[77] The public's response to her appearance was lukewarm.[78] Meanwhile, the House of Commons had forbidden all appeals to Rome and exacted the penalties of praemunire against all who introduced papal bulls into England. It was only then that Pope Clement at last took the step of announcing a provisional sentence of excommunication against the King and Cranmer. He condemned the marriage to Anne, and in March 1534, he declared the marriage to Catherine legal and again ordered Henry to return to her.[79] Henry now required his subjects to swear the oath attached to the First Succession Act, which effectively rejected papal authority in legal matters and recognised Anne Boleyn as queen. Those who refused, such as Sir Thomas More, who had resigned as Lord Chancellor, and John Fisher, Bishop of Rochester, were then placed in the Tower of London. In late 1534 parliament declared Henry "the only supreme head on earth of the Church of England".[80] The Church in England was now under Henry's control, not Rome's. On 14 May 1534, in one of the realm's first official acts protecting Protestant Reformers, Anne wrote a letter to Thomas Cromwell seeking his aid in ensuring that English merchant Richard Herman be reinstated a member of the merchant adventurers in Antwerp and no longer persecuted simply because he had helped in "setting forth of the New testament in English."[81] Before and after her coronation Anne protected and promoted evangelicals and those wishing to study the scriptures of William Tyndale.[82] She had a decisive role in influencing the Protestant reformer Matthew Parker to attend court as her chaplain, and prior to her death entrusted her daughter to Parker's care.[83]

According to author and Tudor historian Alison Weir, Thomas Cromwell plotted Anne's downfall while feigning illness and detailing the plot 20–21 April 1536. Anne's biographer Eric Ives, among others, believes that her fall and execution were engineered by Thomas Cromwell.[106] The conversations between Chapuys and Cromwell thereafter indicate Cromwell as the instigator of the plot to remove Anne; evidence of this is seen in the Spanish Chronicle and through letters written from Chapuys to Charles V. Anne argued with Cromwell over the redistribution of Church revenues and over foreign policy. She advocated that revenues be distributed to charitable and educational institutions; and she favoured a French alliance. Cromwell insisted on filling the King's depleted coffers, while taking a cut for himself, and preferred an imperial alliance.[107] For these reasons, Ives suggests, "Anne Boleyn had become a major threat to Thomas Cromwell."[108] Cromwell's biographer John Schofield, on the other hand, contends that no power struggle existed between Anne and Cromwell and that "not a trace can be found of a Cromwellian conspiracy against Anne... Cromwell became involved in the royal marital drama only when Henry ordered him onto the case."[109] Cromwell did not manufacture the accusations of adultery, though he and other officials used them to bolster Henry's case against Anne.[110] Historian Retha Warnicke questions whether Cromwell could have manipulated the king in such a matter.[111] Henry himself issued the crucial instructions: his officials, including Cromwell, carried them out.[112] The result, historians agree, was a legal travesty.[113] In order to do so the Master Secretary Cromwell would need sufficient evidence that would be convincing enough for her conviction or risk his own offices and perhaps life. Towards the end of April a Flemish musician in Anne's service named Mark Smeaton was arrested. He initially denied being the Queen's lover but later confessed, perhaps tortured or promised freedom. Another courtier, Sir Henry Norris, was arrested on May Day, but being an aristocrat, could not be tortured. Prior to his arrest, Norris was treated kindly by the King, who offered him his own horse to use on the May Day festivities. It seems likely that during the festivities the King was notified of Smeaton's confession and it was shortly thereafter the alleged conspirators were arrested upon his orders. Norris denied his guilt and swore that Queen Anne was innocent; one of the most damaging pieces of evidence against Norris was an overheard conversation with Anne at the end of April, where she accused him of coming often to her chambers not to pay court to her lady-in-waiting Madge Shelton but to herself. Sir Francis Weston was arrested two days later on the same charge, as was Sir William Brereton, a Groom of the King's Privy Chamber. Sir Thomas Wyatt, a poet and friend of the Boleyns who was allegedly infatuated with her before her marriage to the king, was also imprisoned for the same charge but later released, most likely due to his or his family's friendship with Cromwell. Sir Richard Page was also accused of having a sexual relationship with the Queen, but he was acquitted of all charges after further investigation could not implicate him with Anne.[citation needed] The final accused was Queen Anne's own brother, arrested on charges of incest and treason, accused of having a sexual relationship with his sister.[114] George Boleyn was accused of two incidents of incest: November, 1535 at Whitehall and the following month at Eltham.[115] On 2 May 1536 Anne was arrested and taken to the Tower of London by barge. It is likely that Anne may have entered through The Court Gate in The Byward Tower rather than The Traitor's Gate. In the Tower, she collapsed, demanding to know the location of her father and "swete broder", as well as the charges against her. In what is reputed to be her last letter to King Henry, dated May 6, she wrote: "Sir, Your Grace's displeasure, and my imprisonment are things so strange unto me, as what to write, or what to excuse, I am altogether ignorant. Whereas you send unto me (willing me to confess a truth, and so obtain your favour) by such an one, whom you know to be my ancient professed enemy. I no sooner received this message by him, than I rightly conceived your meaning; and if, as you say, confessing a truth indeed may procure my safety, I shall with all willingness and duty perform your demand. But let not your Grace ever imagine, that your poor wife will ever be brought to acknowledge a fault, where not so much as a thought thereof preceded. And to speak a truth, never prince had wife more loyal in all duty, and in all true affection, than you have ever found in Anne Boleyn: with which name and place I could willingly have contented myself, if God and your Grace's pleasure had been so pleased. Neither did I at any time so far forget myself in my exaltation or received Queenship, but that I always looked for such an alteration as I now find; for the ground of my preferment being on no surer foundation than your Grace's fancy, the least alteration I knew was fit and sufficient to draw that fancy to some other object. You have chosen me, from a low estate, to be your Queen and companion, far beyond my desert or desire. If then you found me worthy of such honour, good your Grace let not any light fancy, or bad council of mine enemies, withdraw your princely favour from me; neither let that stain, that unworthy stain, of a disloyal heart toward your good grace, ever cast so foul a blot on your most dutiful wife, and the infant-princess your daughter. Try me, good king, but let me have a lawful trial, and let not my sworn enemies sit as my accusers and judges; yea let me receive an open trial, for my truth shall fear no open flame; then shall you see either my innocence cleared, your suspicion and conscience satisfied, the ignominy and slander of the world stopped, or my guilt openly declared. So that whatsoever God or you may determine of me, your grace may be freed of an open censure, and mine offense being so lawfully proved, your grace is at liberty, both before God and man, not only to execute worthy punishment on me as an unlawful wife, but to follow your affection, already settled on that party, for whose sake I am now as I am, whose name I could some good while since have pointed unto, your Grace being not ignorant of my suspicion therein. But if you have already determined of me, and that not only my death, but an infamous slander must bring you the enjoying of your desired happiness; then I desire of God, that he will pardon your great sin therein, and likewise mine enemies, the instruments thereof, and that he will not call you to a strict account of your unprincely and cruel usage of me, at his general judgment-seat, where both you and myself must shortly appear, and in whose judgment I doubt not (whatsoever the world may think of me) mine innocence shall be openly known, and sufficiently cleared. My last and only request shall be, that myself may only bear the burden of your Grace's displeasure, and that it may not touch the innocent souls of those poor gentlemen, who (as I understand) are likewise in strait imprisonment for my sake. If ever I found favour in your sight, if ever the name of Anne Boleyn hath been pleasing in your ears, then let me obtain this request, and I will so leave to trouble your Grace any further, with mine earnest prayers to the Trinity to have your Grace in his good keeping, and to direct you in all your actions. From my doleful prison in the Tower, this sixth of May; Your most loyal and ever faithful wife, Anne Boleyn" Four of the accused men were tried in Westminster on 12 May 1536. Weston, Brereton, and Norris publicly maintained their innocence and only the tortured Smeaton supported the Crown by pleading guilty. Three days later, Anne and George Boleyn were tried separately in the Tower of London. She was accused of adultery, incest, and high treason.[116] By the Treason Act of Edward III, adultery on the part of a queen was a form of treason (presumably because of the implications for the succession to the throne) for which the penalty was hanging, drawing and quartering for a man and burning alive for a woman, but the accusations, and especially that of incestuous adultery, were also designed to impugn her moral character. The other form of treason alleged against her was that of plotting the king's death, with her "lovers", so that she might later marry Henry Norris.[115] Henry Percy, 6th Earl of Northumberland sat on the jury that found Anne guilty. When the verdict was announced, he collapsed and had to be carried from the courtroom. He died eight months later, leaving no heirs and therefore was succeeded by his nephew. On 14 May, Cranmer declared Anne's marriage to Henry dissolved.[117]

She knelt upright, in the French style of executions. Her final prayer consisted of her repeating continually, "To Jesus Christ I commend my soul; Lord Jesus receive my soul." Her ladies removed her headdress and necklaces, and then tied a blindfold over her eyes. According to Eric W. Ives, the executioner Rombaud was so taken by Anne that he was shaken. Rombaud found it so difficult to proceed that to distract her and for her to position her head correctly, he is said to have shouted, "Where is my sword?" just before killing her.[125][126] The execution consisted of a single stroke.[127] It was witnessed by Thomas Cromwell, Charles Brandon, 1st Duke of Suffolk, the King's illegitimate son, Henry Fitzroy, the Lord Mayor of London, as well as aldermen, sheriffs, and representatives of the various craft guilds. Most of the King's Council were also present.[128] Cranmer, who was at Lambeth Palace, was reported to have broken down in tears after telling Alexander Ales: "She who has been the Queen of England on earth will today become a Queen in heaven."[129] When the charges were first brought against Anne, Cranmer had expressed his astonishment to Henry and his belief that "she should not be culpable." Still, Cranmer felt vulnerable because of his closeness to the queen, and so on the night before the execution, he declared Henry's marriage to Anne to have been void, like Catherine's before her. He made no serious attempt to save Anne's life, although some sources record that he had prepared her for death by hearing her last private confession of sins, in which she had stated her innocence before God.[130] However, on the day of her death a Scottish friend found Cranmer weeping uncontrollably in his London gardens, saying that he was sure that Anne had now gone to Heaven.[131] Henry failed to organise any kind of funeral or even provide a proper coffin for her.[citation needed] Her body lay on the scaffold for some time before a man (believed to be working inside the Tower) found an empty arrow chest and placed her head and body inside.[citation needed] She was then buried in an unmarked grave in the Chapel of St Peter ad Vincula. Her skeleton was identified during renovations of the chapel in the reign of Queen Victoria and Anne's resting place is now marked in the marble floor.